Culture Media

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Eosin Methylene Blue (EMB) agar is a both selective and differential culture medium. It is selective culture medium for Gram-negative bacteria.

STARCH AGAR is used for detection of starch hydrolysing microorganism. This medium was formulated by Vedder in 1915. Although the medium was originally formulated to perform the test for the identification of Bacillus cereus, it can be applied to any kind of microorganism where starch hydrolysis activity is required to be analyzed.

DECARBOXYLASE TEST MEDIUM BASE, FALKOW is used for cultivation and differentiation of bacteria based on their decarboxlase activity. “Falkow” developed the lysine decarboxylase medium for differentiating Salmonellae and Shigellae. This medium contains Yeast extract and Peptic digest of animal tissue which provides nitrogenous nutrients for the growth of bacteria.

BISMUTH SULPHITE AGAR is used for the selective isolation of Salmonella spp. Salmonella constitute the most taxonomically complex group of bacteria among Enterobacteriaceae. Bismuth Sulphite Agar is a modification of Wilson and Blair formula.

Triple Sugar Iron Agar was originally proposed by Sulkin and Willet and modified by Hajna for identifying Enterobacteriaceae. Triple Sugar Iron Agar is recommended for confirmation of gram negative bacilli on basis of dextrose, lactose and sucrose fermentation and H2S production.

Luria Bertani Broth, Miller is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms. Suspend 25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely.

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Simmons Citrate Agar is highly selective for the isolation of Vibrio cholerae and V. parahaemolyticus from stool specimens, and specified in standard methods for food testing. This highly selective medium meets the nutritional requirements of Vibrio species and allows competing with intestinal flora. Vibrio species is able to grow in media containing increased salt concentrations. Vibrio species are natural inhabitants of water.

Macconkey Agar is used for the isolation, enumeration and enrichment of Enterobacteriaceae. MacCONKEY AGAR (as per USP/EP/BP/JP) is used for the isolation, enumeration and enrichment of Enterobacteriaceae. The medium for bacteria designed to selectively isolate Gram-negative and enteric (normally found in the intestinal tract) bacilli and differentiate them based on lactose

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Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation, while inhibiting gram-positive bacteria. Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo was successful in inhibiting gram-positive bacteria on his medium by the incorporation of sodium sulphite and basic fuchsin. The

TELLURITE BLOOD AGAR BASE is used for selective isolation and cultivation of Corynebacterium species. It is selective due to the presence of inhibitor and differential by means of ability of organism to reduce potassium tellurite. Corynebacterium is a genus of gram-positive, facultatively anaerobic, non-motile bacteria that exhibits a fermentative metabolism (carbohydrates to lactic acid) under certain conditions. Many species of Corynebacteria can be isolated from various places such as soil, water, blood, and human skin. Pathogenic strains of Corynebacteria can infect plants, animals, or humans.

Flood the surface of 48 hours old culture on Starch Agar with Grams Iodine (TBL 034). Starch hydrolysis is seen as a colourless zone surrounding the colonies. A blue or purple zone indicates that starch is not hydrolyzed. Size of the clear zone is directly proportional to the starch hydrolyzing activity of the strain under study.