High-throughput screening (HTS) remains the key approach for finding hits and leads in the field of drug discovery. As the number of compounds available for screening has increased dramatically, industrialization and automation of the process have improved the ability to deal with larger numbers of compounds.
https://drug-discovery.creative-biostructure.com/maghelix-hts-assay-development-p30
HTS is a technology system that combines a variety of technical approaches, usually involving robotics, liquid handling devices, detectors, and specialized software for instrument control and data processing. It is based on molecular and/or cell-based experimental methods and is performed in the form of microplates as experimental tools.
https://drug-discovery.creative-biostructure.com/high-throughput-screening-hts-p31
Virtual screening is an approach to select some promising compounds from compound databases for further experimental activity evaluation based on drug design theory and with the help of computer technology and professional application software. Its purpose is to screen out novel leads from dozens or even millions of molecules.
https://drug-discovery.creative-biostructure.com/in-silico-virtual-screening-p32
Ligand-based virtual screening (LBVS) is a good option for hit identification if the three-dimensional (3D) structure of the target is unknown or if it is challenging to perform virtual drug screening through structure-based methods. The LBVS strategy is based on the assumption that molecules with similar structures (e.g., chemical structures, pharmacophores, molecular fields) tend to have similar properties and functions.
https://drug-discovery.creative-biostructure.com/ligand-based-virtual-screening-p37
Fragment-based screening (FBS) is a widely utilized strategy for the discovery of lead compounds. Especially in the development of drugs against unconventional targets such as protein-protein interactions (PPIs), FBS has proven to be a viable alternative to high-throughput screening (HTS).
https://drug-discovery.creative-biostructure.com/fragment-based-screening-fbs-p41
After going through a drug discovery phase such as high-throughput screening (HTS), it is crucial to obtain more information to guide the drug candidate selection and optimization for the next step. Biophysical assays bring a lot of valuable information for characterization and prioritization of hit compounds.
https://drug-discovery.creative-biostructure.com/hit-biophysical-characterization-p45
De novo drug design is a computational technique wherein novel molecular structures with desired pharmacological properties are generated from the beginning or scratch. This technique is complementary to high-throughput screening and in silico virtual screening and is believed to contribute to the pharmaceutical development of novel drugs with desired properties at an extremely low cost and time-efficient manner.
https://drug-discovery.creative-biostructure.com/de-novo-drug-design-p24
Creative Biostructure provides a variety of experimental structural biology approaches to help elucidate the interaction mechanisms between proteins and other molecules (such as proteins, nucleic acids, small molecule compounds, peptides, etc.). At the same time, we can also use computer-based molecular docking methods to predict the three-dimensional (3D) structures of these interacting partners.
https://drug-discovery.creative-biostructure.com/molecular-docking-p39
Lead discovery and optimization play a crucial role to determine the quality of clinical candidates. After this process, a set of compounds can be screened that interact with the target and the confirmed hits have specific activity against the target. Creative Biostructure performs comprehensive lead discovery and optimization solutions including library construction.
https://drug-discovery.creative-biostructure.com/lead-discovery-and-optimization-p19
Our molecular cloning service includes the design and preparation of expression constructs. We provide customers with all commercially available vectors for construct design. Various purification tags are supplied for constructing recombinant plasmids, such as SUMO, MBP, GST, His tags, etc.
https://drug-discovery.creative-biostructure.com/maghelix-gene-to-protein-and-gene-to-structure-p15
