Creative Biostructure offers from the single package to the full range of membrane protein service, starting from DNA cloning to the determination of protein structure. Collaborating with academic organizations, we have optimized Lipidic Cubic Phase (LCP) crystallization method to increase the speed and chance of success.
https://www.creative-biostructure.com/lipidic-cubic-phase-crystallization-472.htm
Membrane proteins, such as G protein-coupled receptors (GPCRs) and ion channels, are important drug targets. However, structure-based drug discovery (SBDD) is hampered by the lack of the corresponding 3D protein models, mostly due to the difficulty of crystallizing membrane proteins in their native state.
https://www.creative-biostructure.com/crystallization-chaperone-strategies-21.htm
Growth of high-quality crystals is a major obstacle in many structural investigations. In recent years, the techniques for screening crystals have improved dramatically. Creative Biostructure is pioneer in the field of crystals screening, such as peptide crystallization and has led many successful campaigns to produce the crystals in small and large scale.
https://www.creative-biostructure.com/Batch-Screening-for-Crystals-Production-587.htm
Obtaining diffraction-quality crystals is a prerequisite for solving any macromolecular structure through X-ray diffraction approaches. Once the appropriate crystals are obtained, it is necessary to submit them to the process of X-ray diffraction data acquisition.
https://www.creative-biostructure.com/x-ray-diffraction-data-collection.htm
Electron Microscopy (EM) has become an extremely popular method for the ultrastructural study of macromolecules, cells, and tissues. An aqueous biological sample is frozen rapidly and irradiated with a beam of electrons. A detector senses how the electrons are scattered, and a computer reconstructs the 3D-shape of the molecule.
https://www.creative-biostructure.com/cryo-em-services_4.htm
Cryo-electron tomography (cryo-ET) is a technique enabling the visualization of frozen hydrated biological samples intact from harmful preparation process. Cryo-ET uses an electron microscope to record a series of 2D projection images acquired by tilting a biological sample held at cryogenic temperature.
https://www.creative-biostructure.com/Cryo-Electron-Tomography-592.htm
Creative Biostructure enables to advance 3D cryo-electron microscopy (cryo-EM) into antigen-antibody complex applications for drug discovery. 3D cryo-EM enables the structure determination of large protein complexes like antigen-antibody complexes, or membrane proteins.
https://www.creative-biostructure.com/Cryo-EM-for-Antigen-antibody-Complex-595.htm
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome, making them non-infectious and safe to handle. The expression of viral structural proteins, such as envelope or capsid can result in their self-assembly into VLPs.
https://www.creative-biostructure.com/cryo-em-for-virus-like-particles-575.htm
Creative Biostructure is a biotechnology company offering drug discovery contract services to pharma, biotech, chemical companies, and academic institutes. Based on our zebrafish screening platform, we can provide MagHelix™ toxicity assays using zebrafish. Zebrafish has been a prominent model vertebrate for investigating chemical toxicity.
https://drug-discovery.creative-biostructure.com/maghelix-toxicity-assays-using-zebrafish-p35
Various nuclear magnetic resonance (NMR) spectroscopy applications have been developed for the identification and validation of hit compounds and lead optimization.
https://drug-discovery.creative-biostructure.com/maghelix-saturation-transfer-difference-std-nmr-p47
In the early stage of drug discovery, the kinetic and thermodynamic characterization of ligands that bind to specific target proteins can provide vital information for the identification and optimization of hit or lead compounds.
https://drug-discovery.creative-biostructure.com/maghelix-isothermal-titration-calorimetry-itc-p51
In addition to isothermal titration calorimetry (ITC), another microcalorimetry technique utilized to study protein-ligand interactions is differential scanning calorimetry (DSC). ITC measures the heat generated or consumed after titration of a ligand onto a target protein (or the reverse).
https://drug-discovery.creative-biostructure.com/maghelix-differential-scanning-calorimetry-dsc-p52
Microscale thermophoresis (MST) has become a common technique to detect specific target-probe interactions, and it measures the differences in the movement rate through a microscopic temperature gradient caused when complexes are formed.
https://drug-discovery.creative-biostructure.com/maghelix-microscale-thermophoresis-mst-p54
Given the significant development in mass spectrometry (MS) related software and hardware, some approaches based on mass spectrometry have been used in the drug discovery, for example, non-covalent mass spectrometry (NC-MS, also called native MS, usually using electrospray ionization mass spectrometry, ESI-MS),
https://drug-discovery.creative-biostructure.com/maghelix-mass-spectrometry-ms-p56
In drug discovery, biophysical approaches have become attractive screening techniques. They are not only the main primary hit finding methodologies, such as weakly active fragments, but also as orthogonal approaches for the validation of hit compounds found by conventional biochemical or cellular assays.
https://drug-discovery.creative-biostructure.com/maghelix-thermal-shift-assay-tsa-p48
For any project that involves determining the structure of biological macromolecules, Creative Biostructure can provide flexible MagHelix™ Gene-to-Protein and Gene-to-Structure solutions.
https://drug-discovery.creative-biostructure.com/maghelix-co-crystallization-and-soaking-p46
