candyjkswift
Creative Biolabs employs phage-display technology to discover new agonist antibodies. Generally, either an immunized phage display library or a naive library will be used for antibody isolation on the recombinant extracellular domains (ECD) of the receptors. Antibodies specific for the receptor ECDs are detected by phage ELISA and the different scFv antibodies are further screened for agonism in some cell-based assays.
By merging the ultralong CDR3s discovered in bovine with human IgG scaffolds, novel therapeutic antibodies against challenging hot antigen families, including GPCRs and other intra-membrane targets on the cell surface are possible. Humanized IgG scaffolds that accommodate ultralong CDR3s may target small proteins with desirable therapeutic properties, which are challenging for conventional antibodies.
